Scope

Our group is focused on uncovering the fundamental cell biology that enables precise protein sorting to the correct subcellular compartments. Cells rely on these dynamic membrane-trafficking pathways to ensure that proteins and lipids reach their proper destinations. We explore the molecular machinery that recognises, sorts and transports cargo through secretory and endosomal routes, with the ultimate aim of understanding how mis-sorting contributes to human disease.

We dissect key trafficking modules—such as adaptor protein complexes, retromer, and exocyst —using an integrated toolkit of high-resolution live-cell imaging (RUSH assays, correlative light and electron microscopy), quantitative proteomics and lipidomics, targeted CRISPR/Cas9 screens, single particle light microscopy and structural biology. These approaches allow us to capture the kinetics and regulation of processes such as Golgi-to-plasma-membrane transport, endosomal recycling, Golgi organisation and related pathways in living cells.

We also use a diverse array of cell types to work understand fundamental processes in physiological context, these include adipocytes, human neurons, and plasma cells.

Defects in these sorting systems underlie neurodegenerative disorders (for example, Parkinson’s and Alzheimer’s), immunodeficiencies and cancer. In close collaboration with clinical and computational partners, we aim to translate our mechanistic insights into potential therapeutic strategies. Through this work, we aim to uncover general principles of subcellular organisation and trafficking that maintain cellular homeostasis and drive disease.

FUNDING

We are greatful to our funders the Wellcome Trust, the Royal Society, the Newton Trust and the BBSRC.